GSE14452

Platform: HG-Focus

Organism: Homo sapiens

Pdf version of the dendrogram

Set title:

DNA microarray analyses for HepG2 cells exposed to silver nanoparticles

Citation:

Kawata K, Osawa M, Okabe S. In vitro toxicity of silver nanoparticles at noncytotoxic doses to HepG2 human hepatoma cells. Environ Sci Technol. 2009 Aug 1;43(15):6046-51.

Set summary:

From the result of the gene expression analyses of human hepatoma cell line, HepG2, a number of genes associated with cell proliferation and DNA repair were distinctively up-regulated by Ag-nanoparticle exposure, suggesting that Ag-nanoparticles might stimulate cell proliferation and DNA damage, which are considered to be mechanisms playing an important role for carcinogenesis and tumor progression. The inductions of these genes involved in cell proliferation were also observed in PS-nanoparticles and Ag2CO3-exposed cells. In addition, the inductions of DNA repair-associated genes were also observed in Ag2CO3-exposure. These results suggest that both “nanoshape” and “silver” can cause the inductions of these gene expression patterns. Furthermore, cysteine, a strong ionic silver ligand partially abolished these gene expressions induced by silver nanoparticles. Ionic silver sourced from Ag-nanoparticles could not fully explain these gene expressions.


Sample_name Description Type Treatment Dose NP_size
GSM361148 HepG2_control rep4 HepG2 control - -
GSM361149 HepG2_control rep5 HepG2 control - -
GSM361150 HepG2_control rep6 HepG2 control - -
GSM361151 HepG2_nanoAg rep1 HepG2 Ag-NP 1mg/L 7-10 nm
GSM361152 HepG2_nanoAg rep2 HepG2 Ag-NP 1mg/L 7-10 nm
GSM361153 HepG2_nanoAg rep3 HepG2 Ag-NP 1mg/L 7-10 nm
GSM361154 HepG2_PS rep1 HepG2 PS-NP 1mg/L 15 nm
GSM361155 HepG2_PS rep2 HepG2 PS-NP 1mg/L 15 nm
GSM361156 HepG2_PS rep3 HepG2 PS-NP 1mg/L 15 nm
GSM361157 HepG2_Ag2CO3 rep1 HepG2 Ag2CO3 1.3 mg/L -
GSM361158 HepG2_Ag2CO3 rep2 HepG2 Ag2CO3 1.3 mg/L -
GSM361159 HepG2_Ag2CO3 rep3 HepG2 Ag2CO3 1.3 mg/L -
GSM361160 HepG2_nanoAg+cysteine rep1 HepG2 Ag-NP + cysteine 1mg/L Ag-NPs + 5mM N-acetyl-L-cysteine 7-10 nm
GSM361161 HepG2_nanoAg+cysteine rep2 HepG2 Ag-NP + cysteine 1mg/L Ag-NPs + 5mM N-acetyl-L-cysteine 7-10 nm
GSM361162 HepG2_nanoAg+cysteine rep3 HepG2 Ag-NP + cysteine 1mg/L Ag-NPs + 5mM N-acetyl-L-cysteine 7-10 nm

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