GSE8608

Platform: HG-U133_Plus_2

Organism: Homo sapiens

Pdf version of the dendrogram

Set title:

Transcription profiling of human monocyte derived macrophages from COPD patients and healthy subjects after treatment with LPS or fine and ultrafine particles

Citation:

Eder C, Frankenberger M, Stanzel F, Seidel A, Schramm KW, Ziegler-Heitbrock L, Hofer TP: Ultrafine carbon particles down-regulate CYP1B1 expression in human monocytes. Part Fibre Toxicol. 2009 Oct 16;6:27.

Set summary:

In this study gene expression of monocyte-derived macrophages (MDM) from chronic obstructive pulmonary disease (COPD) patients and healthy subjects was investigated. MDM were treated with LPS, a combination of fine TiO2 and ultrafine Printex90 particles, or remained untreated. Experiment Overall Design: MDM of 13 COPD patients and 13 healthy subjects were incubated for four hours with LPS (10ng/ml), a combination of fine TiO2 and ultrafine Printex90 (32ug/ml each) or remained untreated. Cells were harvested, counted, and total RNA was isolated (phenol-chloroform extraction) for each subject individually. Total RNA of the 13 individuals from each group (COPD, healthy) and incubation (untreated, LPS, particles) were pooled and hybridized on a seperate array.


Sample_name Description Type Treatment Dose NP_size
GSM213464 humanMDM_COPD_untreated monocyte-derived macrophages - - -
GSM213465 humanMDM_COPD_LPS monocyte-derived macrophages LPS - -
GSM213466 humanMDM_COPD_Particles monocyte-derived macrophages TiO2 and Printex90 32ug/ml each NP NA
GSM213500 humanMDM_Healthy_untreated monocyte-derived macrophages - - -
GSM213511 humanMDM_Healthy_LPS monocyte-derived macrophages LPS - -
GSM213512 humanMDM_Healthy_Particles monocyte-derived macrophages TiO2 and Printex90 32ug/ml each NP NA

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