GSE39316-MDDC

Platform: Illumina Sentrix HumanHT-12 Expression BeadChips, version 3

Organism: Homo sapiens

Pdf version of the dendrogram

Set title:

Nanotoxicogenomic study of ZnO and TiO2 responses

Citation:

Tuomela S, Autio R, Buerki-Thurnherr T, Arslan O et al. Gene expression profiling of immune-competent human cells exposed to engineered zinc oxide or titanium dioxide nanoparticles. PLoS One. 2013 Jul 22;8(7):e68415.

Set summary:

A comprehensive in vitro assessment of two commercial metal oxide nanoparticles, TiO2 and ZnO, was performed using human monocyte-derived macrophages (HMDM), monocyte-derived dendritic cells (MDDC), and T cell leukemia-derived cell line (Jurkat). TiO2 nanoparticles were found to be non-toxic whereas ZnO nanoparticles caused dose-dependent cell death. Subsequently, global gene expression profiling was performed to identify signaling pathways underlying the cytotoxicity caused by ZnO nanoparticles. Analysis was done with doses, 1µg/ml and 10µg/ml after 6 and 24 hours of exposure. Interestingly, 2703 genes were significantly differentially expressed in HMDM upon exposure to 10µg/ml ZnO nanoparticles, while in MDDCs only 12 genes were affected. In Jurkat cells, 980 genes were differentially expressed. It is noteworthy that the gene expression of metallothioneins was upregulated in all the three cell types. In addition to the common ZnO-inducible changes, a notable proportion of the genes were regulated in a cell type-specific manner. Using a panel of ZnO nanoparticles, we obtained an additional support that the cellular response to ZnO nanoparticles is caused by particle dissolution. Gene ontology analysis revealed that the top biological processes disturbed in HMDM and Jurkat cells were regulating cell death and growth. In addition, genes controlling immune system development were affected. Bioinformatics assessment showed that the top human disease category associated with ZnO-responsive genes in both HMDM and Jurkat cells was cancer. Overall, the study revealed novel genes and pathways for mediating ZnO nanoparticle-induced toxicity and demonstrated the value of assessing nanoparticle responses through combined transcriptomics and bioinformatics approach.


Sample_name Description Type Treatment Dose NP_size
GSM960767 MDDC_rep1_ctr_6h_0 MDDC no treatment - -
GSM960777 MDDC_rep2_ctr_6h_0 MDDC no treatment - -
GSM960787 MDDC_rep3_ctr_6h_0 MDDC no treatment - -
GSM960769 MDDC_rep1_TiO22_6h_1 MDDC TiO2 1µg/ml 21nm
GSM960779 MDDC_rep2_TiO2_6h_1 MDDC TiO2 1µg/ml 21nm
GSM960789 MDDC_rep3_TiO2_6h_1 MDDC TiO2 1µg/ml 21nm
GSM960770 MDDC_rep1_TiO2_6h_10 MDDC TiO2 10µg/ml 21nm
GSM960780 MDDC_rep2_TiO2_6h_10 MDDC TiO2 10µg/ml 21nm
GSM960790 MDDC_rep3_TiO2_6h_10 MDDC TiO2 10µg/ml 21nm
GSM960773 MDDC_rep1_ZnO_6h_1 MDDC ZnO 1µg/ml 10 ± 2nm
GSM960783 MDDC_rep2_ZnO_6h_1 MDDC ZnO 1µg/ml 10 ± 2nm
GSM960793 MDDC_rep3_ZnO_6h_1 MDDC ZnO 1µg/ml 10 ± 2nm
GSM960774 MDDC_rep1_ZnO_6h_10 MDDC ZnO 10µg/ml 10 ± 2nm
GSM960784 MDDC_rep2_ZnO_6h_10 MDDC ZnO 10µg/ml 10 ± 2nm
GSM960794 MDDC_rep3_ZnO_6h_10 MDDC ZnO 10µg/ml 10 ± 2nm
GSM960768 MDDC_rep1_ctr_24h_0 MDDC no treatment - -
GSM960778 MDDC_rep2_ctr_24h_0 MDDC no treatment - -
GSM960788 MDDC_rep3_ctr_24h_0 MDDC no treatment - -
GSM960771 MDDC_rep1_TiO2_24h_1 MDDC TiO2 1µg/ml 21nm
GSM960781 MDDC_rep2_TiO2_24h_1 MDDC TiO2 1µg/ml 21nm
GSM960791 MDDC_rep3_TiO2_24h_1 MDDC TiO2 1µg/ml 21nm
GSM960772 MDDC_rep1_TiO2_24h_10 MDDC TiO2 10µg/ml 21nm
GSM960782 MDDC_rep2_TiO2_24h_10 MDDC TiO2 10µg/ml 21nm
GSM960792 MDDC_rep3_TiO2_24h_10 MDDC TiO2 10µg/ml 21nm
GSM960775 MDDC_rep1_ZnO_24h_1 MDDC ZnO 1µg/ml 10 ± 2nm
GSM960785 MDDC_rep2_ZnO_24h_1 MDDC ZnO 1µg/ml 10 ± 2nm
GSM960795 MDDC_rep3_ZnO_24h_1 MDDC ZnO 1µg/ml 10 ± 2nm
GSM960776 MDDC_rep1_ZnO_24h_10 MDDC ZnO 10µg/ml 10 ± 2nm
GSM960786 MDDC_rep2_ZnO_24h_10 MDDC ZnO 10µg/ml 10 ± 2nm
GSM960796 MDDC_rep3_ZnO_24h_10 MDDC ZnO 10µg/ml 10 ± 2nm

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