GSE12405

Platform: Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)

Organism: Homo sapiens

Pdf version of the dendrogram

Set title:

Gene expression of human primary macrophages induced by carbosilane dendrimer 2G-NN16 complexed with or without siRNA

Citation:

Gras R, Almonacid L, Ortega P, Serramia MJ et al. Changes in gene expression pattern of human primary macrophages induced by carbosilane dendrimer 2G-NN16. Pharm Res 2009 Mar;26(3):577-86.

Set summary:

Purpose: The use of dendrimers for biomedical applications has emerged with promising results. 2G-NN16 is a carbosilane dendrimer with sixteen positive charges per molecule tested to be capable to bind and release antisense oligonucleotides (ODNs) and small interference RNA (siRNA) in vitro. In spite of low cytotoxicity observed for these dendrimers, little is known about cellular changes they produce in cells in general and in immune cells in particular. Methods: Genomic technologies allow us to identify global gene expression profile changes in macrophages exposed to a non-toxic concentration (5µM) of 2G-NN16, alone or complexed with a random siRNA (dendriplex). Results were confirmed by quantitative real-time RT-PCR. Results: Exposing macrophages to this dendrimer or dendriplex causes multiple gene expression changes, but no specific action of random siRNA was detected. Pathway analysis of differentially expressed genes shows the altered functions to be immune response, proliferation and transcription regulation. Interleukin 17F (IL17F) was the most regulated gene. Conclusions: Global gene expression profiles are a highly sensitive method to measure the toxicity degree of a gene delivery vehicle. The strong repression of IL17F, IL23R and IL23A, all of them involved in autoimmune disease, by this particular dendrimer suggests a potential pharmacological application.


Sample_name Description Type Treatment Dose NP_size
GSM311264 Macrophage_dendrimer_comparison1 Primary Macrophages 2G-NN16 carbosilane dendrimer 5µM NA
GSM311272 Macrophage_dendrimer_comparison2 Primary Macrophages 2G-NN16 carbosilane dendrimer 5µM NA
GSM311297 Macrophage_dendrimer_comparison3 Primary Macrophages 2G-NN16 carbosilane dendrimer 5µM NA
GSM311298 Macrophage_dendriplex_comparison1 Primary Macrophages 2G-NN16 carbosilane dendrimer complexed with a random siRNA 5µM NA
GSM311299 Macrophage_dendriplex_comparison2 Primary Macrophages 2G-NN16 carbosilane dendrimer complexed with a random siRNA 5µM NA
GSM311306 Macrophage_dendriplex_comparison3 Primary Macrophages 2G-NN16 carbosilane dendrimer complexed with a random siRNA 5µM NA

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