GSE20431

Platform: Operon Human Genome Array-Ready Oligo Set[tm] (AROS) Version 4.0 (GPL7088 and GPL10077)

Organism: Homo sapiens

Pdf version of the dendrogram

Set title:

Gene expression profiling on FePro labeled bone marrow stromal cells

Citation:

Balakumaran A, Pawelczyk E, Ren J, Sworder B et al. Superparamagnetic iron oxide nanoparticles labeling of bone marrow stromal (mesenchymal) cells does not affect their "stemness". PLoS One 2010 Jul 7;5(7):e11462. PMID: 20628641

Set summary:

Superparamagnetic iron oxide nanoparticles (SPION) are increasingly used to label human bone marrow stromal cells (BMSCs, also called mesenchymal stem cells) to monitor their fate by in vivo MRI and histology after Prussian blue (PB) staining. SPION-labeling appears to be safe as assessed by in vitro differentiation of BMSCs, however, we chose to determine the effect of labeling on maintaining the “stemness” of cells within the BMSC population. Colony forming efficiency, CD146 expression, gene expression profiling, bone and myelosupportive stroma formation in vivo were evaluated. SPION-labeling did not alter these assays. Equivalent bone with host hematopoiesis was formed by SPION-labeled and unlabeled BMSCs. PB+ adipocytes were noted, definitively demonstrating their donor origin, as well as PB+ pericytes, indicative of self-renewal of the stem cell in the BMSC population. This study confirms that SPION labeling does not alter the differentiation potential of the subset of stem cells within BMSCs.


Sample_name Description Type Treatment Dose NP_size
GSM307451 LFB - mAdbID:94275 lung fibroblasts - - -
GSM307454 WA09 (H9) - replicate 1 - mAdbID:94280 embryonic stem cells WA09 (H9) - - -
GSM307458 UASMC - mAdbID:94287 Umbilical Artery Smooth Muscle Cells (UASMC) - - -
GSM307462 HUVEC - mAdbID:94292 Human Umbilical Vein Endothelial Cells (HUVEC) - - -
GSM307465 WA09 (H9) - replicate 2 - mAdbID:94793 embryonic stem cells WA09 (H9) - - -
GSM307466 WA09 (H9) - replicate 3 - mAdbID:94794 embryonic stem cells WA09 (H9) - - -
GSM512438 BMSC-gold - repeat 2 - mAdbID:105986 bone marrow stromal cells (BMSCs) Gold Nanoparticle 2.25 x 107 NP/ml 250nm
GSM512439 BMSC-FePro - repeat 2 - mAdbID:105987 bone marrow stromal cells (BMSCs) FePro final 50:3µg/ml -
GSM512440 BMSC-control - repeat 3 - mAdbID:105989 bone marrow stromal cells (BMSCs) - - -
GSM512441 BMSC-gold - repeat 3 - mAdbID:105990 bone marrow stromal cells (BMSCs) Gold Nanoparticle 2.25 x 107 NP/ml 250nm
GSM512442 BMSC-control - repeat 1 - mAdbID:105991 bone marrow stromal cells (BMSCs) - - -
GSM512443 BMSC-FePro - repeat 3 - mAdbID:105992 bone marrow stromal cells (BMSCs) FePro final 50:3µg/ml -
GSM512444 BMSC-control - repeat 2 - mAdbID:105993 bone marrow stromal cells (BMSCs) - - -
GSM512445 BMSC-gold - repeat 1 - mAdbID:105994 bone marrow stromal cells (BMSCs) Gold Nanoparticle 2.25 x 107 NP/ml 250mm
GSM512446 BMSC-FePro - repeat 1 - mAdbID:105995 bone marrow stromal cells (BMSCs) FePro final 50:3µg/ml -

<<back