GSE43515

Platform: Affymetrix GeneChip Human Genome U133 Plus 2.0

Organism: Homo sapiens

Pdf version of the dendrogram

Set title:

Effect of MWCNT on Lung Fibroblast gene expression

Citation:

Set summary:

Multi-walled carbon nanotubes (MWCNT) cause lung fibrosis in rodents and exacerbate airway fibrosis in the mouse ovalbumin model of allergic asthma. Interleukin 13 (IL-13) is a key cytokine secreted by T helper type 2 (Th2) cells. IL-13 is up-regulated in human asthma and animal models that activate pro-fibrotic and pro-proliferative cell signaling cascades in human lung fibroblasts (HLF). This study tested the hypothesis that IL-13 alters the gene expression profile of HLF exposed to MWCNT. Carbon black nanoparticles (CBNP) were also compared to MWCNT as they are relatively inert nanoparticles that do not cause fibrosis. Confluent, quiescent cultures of HLF were treated with 10 ng/ml IL-13 or serum-free defined medium (vehicle) for 24 hours prior to treatment with 10 µg/cm2 MWCNT or CBNP. At 4, 24, or 48 hours following nanoparticle exposure, total RNA was isolated and gene expression was measured using the Affymetrix Human Genome U133A2.0 Array. The data were analyzed using the JMP Genomics statistical platform. IL-13 and MWCNT each caused changes in the expression of distinct gene subsets over the time-course investigated. The combination of IL-13 and MWCNT resulted in a gene expression profile that was distinct from patterns induced or suppressed by either IL-13 or MWCNT alone. CBNP caused changes in gene expression that were distinct from IL-13 or MWCNT. Interestingly, the combination of IL-13 and MWCNT increased the expression of IL-17A and increased collagen (Col1A1), while MWCNT alone increased interferon-inducible protein-27 (IFI27), suggesting that Th2 microenvironment containing IL-13 shifts MWCNT-induced gene expression from a Th1 to a Th17 gene expression profile. These data provide insight into the mechanisms by which MWCNT alter the biology of fibroblasts during normal and allergic inflammatory conditions.


Sample_name Description Type Treatment Dose NP_size
GSM1064718 vehicle control 4 h with IL-13 1__19 lung fibroblasts (16lu) vehicle control with IL-13 10μg/cm2
GSM1064736 vehicle control 4 h with IL-13 19__37 lung fibroblasts (16lu) vehicle control with IL-13 10μg/cm2
GSM1064754 vehicle control 4 h with IL-13 37__55 lung fibroblasts (16lu) vehicle control with IL-13 10μg/cm2
GSM1064719 vehicle control 4 h w/o IL-13 2__20 lung fibroblasts (16lu) vehicle control w/o IL-13 10μg/cm2
GSM1064737 vehicle control 4 h w/o IL-13 20__38 lung fibroblasts (16lu) vehicle control w/o IL-13 10μg/cm2
GSM1064755 vehicle control 4 h w/o IL-13 38__56r lung fibroblasts (16lu) vehicle control w/o IL-13 10μg/cm2
GSM1064720 CBNP 4 h with IL-13 3__21 lung fibroblasts (16lu) CBNP with IL-13 10μg/cm2
GSM1064738 CBNP 4 h with IL-13 21__39 lung fibroblasts (16lu) CBNP with IL-13 10μg/cm2
GSM1064756 CBNP 4 h with IL-13 39__57 lung fibroblasts (16lu) CBNP with IL-13 10μg/cm2
GSM1064721 CBNP 4 h w/o IL-13 4__22 lung fibroblasts (16lu) CBNP w/o IL-13 10μg/cm2
GSM1064739 CBNP 4 h w/o IL-13 22__40 lung fibroblasts (16lu) CBNP w/o IL-13 10μg/cm2
GSM1064757 CBNP 4 h w/o IL-13 40__58 lung fibroblasts (16lu) CBNP w/o IL-13 10μg/cm2
GSM1064722 MWCNT 4 h with IL-13 5__23 lung fibroblasts (16lu) MWCNT with IL-13 10μg/cm2
GSM1064740 MWCNT 4 h with IL-13 23__41 lung fibroblasts (16lu) MWCNT with IL-13 10μg/cm2
GSM1064758 MWCNT 4 h with IL-13 41__59 lung fibroblasts (16lu) MWCNT with IL-13 10μg/cm2
GSM1064723 MWCNT 4 h w/o IL-13 6__24 lung fibroblasts (16lu) MWCNT w/o IL-13 10μg/cm2
GSM1064741 MWCNT 4 h w/o IL-13 24__42 lung fibroblasts (16lu) MWCNT w/o IL-13 10μg/cm2
GSM1064759 MWCNT 4 h w/o IL-13 42__60 lung fibroblasts (16lu) MWCNT w/o IL-13 10μg/cm2
GSM1064724 vehicle control 24 h with IL-13 7__25 lung fibroblasts (16lu) vehicle control with IL-13 10μg/cm2
GSM1064742 vehicle control 24 h with IL-13 25__43 lung fibroblasts (16lu) vehicle control with IL-13 10μg/cm2
GSM1064760 vehicle control 24 h with IL-13 43__61 lung fibroblasts (16lu) vehicle control with IL-13 10μg/cm2
GSM1064725 vehicle control 24 h w/o IL-13 8__26 lung fibroblasts (16lu) vehicle control w/o IL-13 10μg/cm2
GSM1064743 vehicle control 24 h w/o IL-13 26__44 lung fibroblasts (16lu) vehicle control w/o IL-13 10μg/cm2
GSM1064761 vehicle control 24 h w/o IL-13 44__62 lung fibroblasts (16lu) vehicle control w/o IL-13 10μg/cm2
GSM1064726 CBNP 24 h with IL-13 9__27 lung fibroblasts (16lu) CBNP with IL-13 10μg/cm2
GSM1064744 CBNP 24 h with IL-13 27__45 lung fibroblasts (16lu) CBNP with IL-13 10μg/cm2
GSM1064762 CBNP 24 h with IL-13 45__63 lung fibroblasts (16lu) CBNP with IL-13 10μg/cm2
GSM1064727 CBNP 24 h w/o IL-13 10__28 lung fibroblasts (16lu) CBNP w/o IL-13 10μg/cm2
GSM1064745 CBNP 24 h w/o IL-13 28__46 lung fibroblasts (16lu) CBNP w/o IL-13 10μg/cm2
GSM1064763 CBNP 24 h w/o IL-13 46__64 lung fibroblasts (16lu) CBNP w/o IL-13 10μg/cm2
GSM1064728 MWCNT 24 h with IL-13 11__29 lung fibroblasts (16lu) MWCNT with IL-13 10μg/cm2
GSM1064746 MWCNT 24 h with IL-13 29__47 lung fibroblasts (16lu) MWCNT with IL-13 10μg/cm2
GSM1064764 MWCNT 24 h with IL-13 47__65 lung fibroblasts (16lu) MWCNT with IL-13 10μg/cm2
GSM1064729 MWCNT 24 h w/o IL-13 12__30 lung fibroblasts (16lu) MWCNT w/o IL-13 10μg/cm2
GSM1064747 MWCNT 24 h w/o IL-13 30__48 lung fibroblasts (16lu) MWCNT w/o IL-13 10μg/cm2
GSM1064765 MWCNT 24 h w/o IL-13 48__66 lung fibroblasts (16lu) MWCNT w/o IL-13 10μg/cm2
GSM1064730 vehicle control 48 h with IL-13 13__31 lung fibroblasts (16lu) vehicle control with IL-13 10μg/cm2
GSM1064748 vehicle control 48 h with IL-13 31__49 lung fibroblasts (16lu) vehicle control with IL-13 10μg/cm2
GSM1064766 vehicle control 48 h with IL-13 49__67 lung fibroblasts (16lu) vehicle control with IL-13 10μg/cm2
GSM1064731 vehicle control 48 h w/o IL-13 14__32 lung fibroblasts (16lu) vehicle control w/o IL-13 10μg/cm2
GSM1064749 vehicle control 48 h w/o IL-13 32__50 lung fibroblasts (16lu) vehicle control w/o IL-13 10μg/cm2
GSM1064767 vehicle control 48 h w/o IL-13 50__68 lung fibroblasts (16lu) vehicle control w/o IL-13 10μg/cm2
GSM1064732 CBNP 48 h with IL-13 15__33 lung fibroblasts (16lu) CBNP with IL-13 10μg/cm2
GSM1064750 CBNP 48 h with IL-13 33__51 lung fibroblasts (16lu) CBNP with IL-13 10μg/cm2
GSM1064768 CBNP 48 h with IL-13 51__69 lung fibroblasts (16lu) CBNP with IL-13 10μg/cm2
GSM1064733 CBNP 48 h w/o IL-13 16__34 lung fibroblasts (16lu) CBNP w/o IL-13 10μg/cm2
GSM1064751 CBNP 48 h w/o IL-13 34__52 lung fibroblasts (16lu) CBNP w/o IL-13 10μg/cm2
GSM1064769 CBNP 48 h w/o IL-13 52__70 lung fibroblasts (16lu) CBNP w/o IL-13 10μg/cm2
GSM1064734 MWCNT 48 h with IL-13 17__35 lung fibroblasts (16lu) MWCNT with IL-13 10μg/cm2
GSM1064752 MWCNT 48 h with IL-13 35__53 lung fibroblasts (16lu) MWCNT with IL-13 10μg/cm2
GSM1064770 MWCNT 48 h with IL-13 53__71 lung fibroblasts (16lu) MWCNT with IL-13 10μg/cm2
GSM1064735 MWCNT 48 h w/o IL-13 18__36 lung fibroblasts (16lu) MWCNT w/o IL-13 10μg/cm2
GSM1064753 MWCNT 48 h w/o IL-13 36__54 lung fibroblasts (16lu) MWCNT w/o IL-13 10μg/cm2
GSM1064771 MWCNT 48 h w/o IL-13 54__72 lung fibroblasts (16lu) MWCNT w/o IL-13 10μg/cm2

<<back